365173 Electrokinetic Particle Sorting By Shape in a Spiral Microchannel

Monday, November 17, 2014
Marquis Ballroom C (Marriott Marquis Atlanta)
Xinyu Lu1, John DuBose1, Shizhi Qian2, Sang Woo Joo3 and Xiangchun Xuan4, (1)Clemson University, Clemson, SC, (2)Department of Mechanical and Aerospace Engineering, Old Dominion University, Norfolk, VA, (3)School of Mechanical Engineering, Yeungnam University, Gyongsan, South Korea, (4)Mechanical Engineering, Clemson University, Clemson, SC

Shape is an intrinsic marker of cell cycle and also a useful indicator of cell state for disease diagnostics. We present in this talk a continuous-flow electrokinetic sorting of spherical and peanut-shaped particles of similar volumes in an asymmetric double-spiral microchannel. It exploits curvature-induced dielectrophoresis (C-iDEP) to focus particles to a tight stream in the first spiral and subsequently displace them to shape-dependent flow paths in the second spiral. We also develop a numerical model to simulate and understand this shape-based particle sorting in spiral microchannels, which predicts the experimental observation qualitatively.

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