365095 Continuous Label-Free Particle Separation Via Wall-Induced Lift in Electrophoresis

Wednesday, November 19, 2014: 2:35 PM
Marquis Ballroom D (Marriott Marquis Atlanta)
Xinyu Lu, Clemson University, Clemson, SC, Cory Thomas, Clemson University, clemson, SC and Xiangchun Xuan, Mechanical Engineering, Clemson University, Clemson, SC

The efficient separation of discrete particle (both synthetic and biological) species is a topic of interest in numerous research fields for its practical application to problems encountered in both academia and industry. We demonstrate in this work a novel continuous-flow label-free method for electrokinetic separation of particles based on size and/or surface charge. This method utilizes the wall-induced non-inertial lift force to deflect a sheath flow-focused particle mixture to property-dependent positions in a straight microchannel. A numerical model is also developed to understand this separation and to study the parametric effects on it. The numerical predictions are found to agree reasonably with the experimental observations.

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See more of this Session: Electrokinetics in Non-Polar Media
See more of this Group/Topical: 2014 Annual Meeting of the AES Electrophoresis Society