358214 Enzymatic Preparation of Steviolbioside from Hydrolysis of Stevioside Catalyzed with a β- Galactosidase

Wednesday, November 19, 2014
Galleria Exhibit Hall (Hilton Atlanta)
Junming Chen, Li Ding and Yongmei Xia, State Key Laboratory of Food Science and Technology, School of Chemistry and material engineering, Jiangnan University, Wuxi, China

Steviolbioside is a terpenoid with biological activity which can be converted to drugs with anti tubereulotie and antibacterial activity. Unfortunately, steviolbioside is a rare component in extract of Stevia rebaudiana Bertoni leaves. In this work, a β-galactosidase from Kluyveromyces lactis presented a strict substrate specifity on converting stevioside to steviolbioside in a stoichiometric yield. It presented specific hydrolytic activity on favors cleavage of glycosyl ester linkage of stevioside. The results showed that the highest stevioside conversion reached to 98.36% accompanying with a steviolbioside yield of 94.88% in 12 h under the optimum conditions.

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See more of this Session: Poster Session: Bioengineering
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division