349887 Protein Adsorption and Cell Growth Responses to P(MEO2MA-co-OEGMA) Thermoresponsive Polymer Brushes

Monday, November 4, 2013
Grand Ballroom B (Hilton)
Matthew Warrener and Christopher Anderson, Chemical and Biomolecular Engineering, Lafayette College, Easton, PA

Smart biomaterials known as thermoresponsive polymers (TRP) have great potential as cell culture substrates due to their temperature dependent surface properties. Specifically, TRPs exhibit a lower critical solution temperature (LCST) that determines the temperature at which the polymers support cell adhesion and proliferation.  The “gold standard” of these TRPs, Poly(N-isopropylacrylamide) (PNIPAM) has been well characterized. However, a relatively new polymer, P(MEO2MA-co-OEGMA), has recently been fabricated that has a significant advantage over PNIPAM: a tunable lower critical solution temperature (LCST). This property allows the thermoswitching temperature to be controlled simply by alternating the ratio of MEO2MA to OEGMA monomers in the polymer synthesis.
        The objective of this study was to synthesize P(MEO2MA-co-OEGMA) polymers and investigate the proteomic responses to the materials and compare those to tissue culture plastic (TCP) and PNIPAM.  The assays used to obtain these measures were absorbance-based assays that examined protein concentration and cell count. It was found that protein adsorption on the new thermobrushes was equal to or greater than TCP and PNIPAM and that as hydrophobicity of the brushes increased, protein adsorption increased, as well. These findings, along with cell adhesion assays and genomic data from a collaborator, will be used in future work to attempt to optimize the growth substrates via selectively pre-adsorbing protein to the brush.

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