349641 Condensing Genomic Information Toward Screening Distal Chromosomal Sites

Monday, November 4, 2013
Grand Ballroom B (Hilton)
George Degen1, Ramsey Ibrahim Zeitoun2, Andrew Garst2 and Dr. Ryan T. Gill2, (1)Chemical Engineering, Carnegie Mellon University, Pittsburgh, PA, (2)Chemical Engineering, University of Colorado, Boulder, CO

As new methods for genomic engineering have been developed, a need for cost effective DNA sequencing has emerged. To improve the rate at which relevant genomic information can be sequenced, a technique for condensing genomic information has been developed. Under this method, targeted sites on the genome of E. coli were condensed into a single compact construct by overlap extension PCR using specially designed primers. A scalable kinetic model was developed and employed to optimize PCR parameters.

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