349537 Developing Novel Selectable Protein Engineering Using Crispr Genome Editing

Monday, November 4, 2013
Grand Ballroom B (Hilton)
E. Hutson Chilton, Bioengineering, Rice University, Houston, TX, Andrew Garst, Chemical Engineering, University of Colorado, Boulder, CO and Ryan T. Gill, Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO

While current methods of protein and metabolic engineering can rapidly introduce many mutations into a population, their lack of selectability leads to a large amount of time and energy spent screening populations to find mutants. We aim to markedly decrease screening load by utilizing the CRISPR/Cas system in Escherichia coli to kill cells which have not acquired the desired mutations. Our goal is to combine this technique with Multiplex Automated Genome Engineering (MAGE) to create a tool for highly efficient selectable protein engineering in vivo.

Extended Abstract: File Not Uploaded