348766 Yeast Metabolic Dynamics for Metabolic Engineering

Monday, November 4, 2013
Grand Ballroom B (Hilton)
Taylor Dolberg1, McKenzie Smith2 and Mark P. Styczynski2, (1)Arizona State University, Tempe, AZ, (2)School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA

Metabolism is the sum of all the chemical reactions in a living organism, and a systems-level understanding of metabolism will facilitate metabolic engineering efforts. Metabolic engineering refers to the control of metabolic activities of an organism to establish and optimize the production of desirable metabolites. Understanding of metabolomics, the analysis of the total metabolic content of the system, is essential to metabolic engineering. The objective of this yeast metabolomics research was to explore the effects of various environmental or genetic perturbations on the metabolite profiles, which data can then be integrated into a mathematical model of yeast metabolism. These effects were probed through four main perturbation experiments: glucose deprivation, galactose substitution, deletion of a metabolic enzyme, and salt challenge. For these experiments, Saccharomyces cerevisiae were grown in batch culture, and a perturbation was applied in log-phase growth. At various time points, culture samples were taken and quenched to stop metabolism, and the cellular metabolites were then extracted, derivatized, and analyzed by GC-MS. The metabolite profiles of the perturbed samples were compared to unperturbed samples grown in corresponding conditions. This information about the systemic metabolic response to perturbation will be used to assist in the construction of yeast metabolism models that may possibly facilitate the production of useful materials from yeast, like biofuels or pharmaceuticals.

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