288576 Bioactive Surfaces in Microfluidic Devices
Microfluidic channels with immobilized proteins and antibodies have been utilized for a wide range of applications, from clinical diagnostics to tissue engineering. This presentation will describe our work in the isolation of stem and progenitor cells for diagnostics and therapeutics from complex samples such as blood and digested tissue. A key element in our approach is the design of alginate hydrogel-based surface coatings that can be applied onto the surfaces of microfluidic channels. These hydrogels are functionalized with antibodies, enabling specific capture of target cells, but more importantly they also have the ability to release captured cells without loss of viability or function. Specific examples to be covered in the presentation will include isolation of endothelial progenitor cells for cardiovascular diagnostics and vascular tissue engineering and isolation of intestinal stem cells for intestinal organoid unit regeneration.