283096 Novel Aspects of the Hydrodynamics of Particle-Laden Liquids: Example of a Successful Transient Flow Immunoassay (TrIA)

Tuesday, October 30, 2012: 2:45 PM
409 (Convention Center )
Anand B. Subramaniam1, Olga Taran1, E. Jane Maxwell1 and George M. Whitesides2, (1)Chemistry and Chemical Biology, Harvard University, Cambridge, MA, (2)Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA

In medicine, measuring the amount of solid precipitate formed when antigens react with antibodies is a foundational principle used to conduct a wide variety of diagnostic tests.  Typically, the degree of precipitation of antigen-antibody complexes is determined by measuring the light scattered off the suspended solids. To obtain accurate measurements of the suspended solids, specialized light sources and detectors are required; thus dedicated and well-calibrated instruments are essential. Here we show that the amount of solids suspended in liquids can be determined using a novel aspect of the hydrodynamics of particle-laden liquids. Using our method, the amount of solids in a liquid can be determined with minute quantities of liquid (< 20 microliters) and short times (< 10 minutes) without the need for dedicated optics and instruments. As an example of a successful application, we conduct an immunoassay to determine the amount of total serum IgG, which is an antibody present in blood.

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See more of this Session: Particulate and Multiphase Flows I
See more of this Group/Topical: Engineering Sciences and Fundamentals