282127 Polymerizable Phospholipid Vesicles

Monday, October 29, 2012
Hall B (Convention Center )
Laurelle Giovannoli, University of Connecticut, Storrs, CT

Advances in medicine introduce more effective treatments but these drugs can be toxic to regular organs.  To decrease this toxicity, phospholipid vesicles can be used to encapsulate the drugs and distributed them through a targeted and controlled release mechanism.  As a result, the circulation half-life (i.e., the in-vivo stability) of carriers plays an important role in the efficacy of drug delivery carriers. A system composed of DHPC (a short-chain lipid), DPPG (a long-chain charged lipid) and DC8,9PC (a polymerizable lipid) has been shown to spontaneously form uniform, nanoscopic polymerizable vesicles.  Presumably, the polymerizable DC8,9PC can enhance the stability of the vesicles. Small angle neutron scattering (SANS) and dynamic light scattering data as well as transmission electron microscopic (TEM) images have confirmed the nanoscopic vesicles.  The TEM images of the polymerized sample show a vesicle-like structure with a rippled irregular edge, which may be associated with tensions created upon polymerization of DC8,9PC.

            It has been reported that a mixture of a 1:1 molar ratio of DNPC (a spacer lipid) to DC8,9PC indicates an increased polymerization of DC8,9PC. We have extended the study on structural characterization of a mixture of DHPC, DPPG, DC8,9PC and DNPC using SANS.  The result shows that incorporation of DNPC further narrows the size distribution of the unilamellar vesicles. The polymerization and stability of this mixture are also studied to provide the fundamental understanding of the effect of DC8,9PC on final morphology.

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