279142 Isolation and Capture of Circulating Tumor Cells by a Microfluidic Device with Functionalized Planar Structures

Thursday, November 1, 2012: 12:48 PM
Washington (Westin )
Hyeun Joong Yoon1, Tae Hyun Kim2, Zhuo Zhang1, Trinh M. Pham1 and Sunitha Nagrath1, (1)Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, (2)Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI

Circulating tumor cells (CTCs) have been identified in the art in peripheral blood from cancer patients and are likely the origin of metastatic disease. Isolation and capture of CTCs represent a potential alternative to invasive biopsies during diagnosis of disease. In the recent past, various microfluidic based CTC isolation technologies have been developed to capture, indentify, sort, and enumerate CTCs. Micro-sized structures functionalized with anti-epithelial-adhesion-molecule antibodies (Anti-EpCAM) is the common approach to capture CTCs. Here we present a microfluidic device with functionalized planar structure to capture CTCs. The microfluidic device has a PDMS chamber and patterned gold posts. It is much easier to scan, count and grow captured cells in comparison with previous micro-sized post-based structure. CTCs were isolated by assembling Graphene oxide on the planar structures for enhanced sensitivity. The functionalized graphene oxide has been used to selectively capture MCF-7 breast cancer cells with a capture rate higher than 90 %.

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See more of this Session: Biosensor Devices II
See more of this Group/Topical: Topical 9: Sensors