277843 Development of an Integrated Peptide for Gene Delivery

Thursday, November 1, 2012: 4:05 PM
Westmoreland Central (Westin )
Qiong Tang, Bin Cao and Gang Cheng, Department of Chemical and Biomolecular Engineering, University of Akron, Akron, OH

To achieve an efficient delivery of therapeutics to physiological targets, the design and/or discovery of effective delivery vehicles play an important role. Polymer-based carriers have been intensively investigated and shown potential clinical applications due to their merits in safety including the avoidance of immunogenicity and the possibility of repeated administration. However, multi-step reaction and purification involved in the synthesis make the purity and fidelity of materials the most challenging issues for large scale preparation of synthetic drug delivery materials. Besides, long-term toxicity and biocompatibility are also concerns for synthetic materials. Peptide-based vectors are gaining prominence in the field of precisely designed and controlled biomaterials. However, these peptide-based materials only occupy partial functions compared to a fully functional drug delivery carrier which is supposed to have drug loading capability and specific targeting. In order to address these challenges in the field of gene delivery, we developed a peptide for DNA delivery. Evaluation of the peptide-based carrier is presented in details, focusing on size and zeta potential of the peptide/DNA complex, blood stability, cytotoxicity and gene transfection efficiency on target cells.

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See more of this Session: Biomaterials for Nucleic Acid Delivery
See more of this Group/Topical: Materials Engineering and Sciences Division