277699 Carbon Nanotube-Based Antimicrobial Biomaterials Formed Via Layer-by-Layer Assembly with Polypeptides

Thursday, November 1, 2012: 4:35 PM
Cambria West (Westin )
Seyma Aslan1, Marie Deneufchatel2, Sara Hashmi1, Nan Li1, Lisa Pfefferle3, Menachem Elimelech4, Emmanuel Pauthe2 and Paul Van Tassel1, (1)Yale University, New Haven, CT, (2)Universite de Cergy-Pontoise, Cergy-Pontoise, France, (3)Chemical Engineering, Yale University, Branford, CT, (4)Department of Chemical and Environmental Engineering, Yale University, New Haven, CT

Biomaterials capable of suppressing microbial infection are of clear importance in various health care applications, e.g. implantable devices. In this study, we investigate the antimicrobial activity of single-walled carbon nanotubes (SWNT) layer-by-layer (LbL) assembled with the polyelectrolytes poly(L-lysine) (PLL) and poly(glutamic acid) (PGA). SWNT dispersion in aqueous solution is achieved through the biocompatible nonionic surfactant polyoxyethylene(20)sorbitan monolaurate (Tween 20), and the amphiphilic polymer phospholipid-poly(ethylene glycol) (PL-PEG). Absorbance spectroscopy and transmission electron microscopy (TEM) show SWNT with either Tween 20 or PL-PEG in aqueous solution to be well dispersed, at about the level of SWNT in chloroform. Quartz crystal microgravimetry with dissipation (QCMD) measurements show both SWNT-Tween and SWNT-PL-PEG to LbL assemble with PLL and PGA into multilayer films, with the PL-PEG system yielding the greater final SWNT content. Escherichia coli and Staphylococcus epidermidis inactivation rates are significantly higher (up to 90%) upon 24 hour incubation with SWNT containing films, compared to control films (ca. 20%). This study demonstrates the potential usefulness of SWNT/PLL/PGA thin films as antimicrobial biomaterials.

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See more of this Session: Nanostructured Biomaterials
See more of this Group/Topical: Materials Engineering and Sciences Division