276240 Genome-Scale Model and Pathway Analysis for Optimization of Butyric Acid Fermentation by Clostridium Tyrobutyricum
276240 Genome-Scale Model and Pathway Analysis for Optimization of Butyric Acid Fermentation by Clostridium Tyrobutyricum
Wednesday, October 31, 2012: 3:15 PM
Washington (Westin )
Understanding the metabolic behavior of an organism or a living cell is essential for fermentation process optimization. Predictions of metabolic behavior and flux distribution in batch cultures enable better regulation of fermentation in terms of biomass yield and productivity. Flux balance analysis (FBA) is a constraint-based modeling approach using linear programming to study cellular metabolism. It can not only develop models based on annotated genomic information, but also predict or validate current knowledge of specific microorganism’s metabolic network. In this study, FBA was performed using Pathway Tool for systematic analysis of butyric acid fermentation by Clostridium tyrobutyricum. A comprehensive genome-scale model was developed for this organism, and flux analysis was then performed to evaluate the constraint factors of fermentation, optimize the process, and predict mutant strain metabolic behavior. The results from this analysis will be presented in this paper.
See more of this Session: Advances in Food and Bioprocess Engineering
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division - See also TI: Comprehensive Quality by Design in Pharmaceutical Development and Manufacture
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division - See also TI: Comprehensive Quality by Design in Pharmaceutical Development and Manufacture