275775 Supramolecular Nano-Beacons As Protease Sensors and Imaging Agents

Thursday, November 1, 2012: 3:55 PM
311 (Convention Center )
Lye Lin Lock, Andrew G. Cheetham, Pengcheng Zhang and Honggang Cui, Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD

Molecular beacons which convert specific chemical reactions or binding events into measurable signals are essential tools for understanding cellular and sub-cellular activities at molecular level. In this context, we successfully designed and synthesized a peptide-based drug conjugated nano-beacon that could serve as a cancer detection tool as well as a carrier for targeted drug delivery in cancer treatment. An important characteristic of nano-beacons is that they can self-assemble into nanostructure under physiological condition, which enhances the cancer-cell permeability and retention (EPR effect).  Here we designed and synthesized a novel nano-beacons structure conjugated with anti-cancer drug and an enzymatically degradable linker. This linker is susceptible to the cleavage of cathepsin-B, a lysosomal enzyme that is over-expressed in many cancerous cell lines, hence allowing the release of drug at cancer site. Our results showed that these cleavable drug-conjugated nano-beacons are uptake by cancerous MCF-7 cells at the lysosome before diffusing into the cell nuclei. The delivery of anti-cancer drug to MCF-7 results in cell apoptosis as well as the decrease in viability after incubation with the drug-conjugated nano-beacon.

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See more of this Session: Nanotechnology for In Vivo and In Vitro Imaging
See more of this Group/Topical: Nanoscale Science and Engineering Forum