269666 Highly Efficient Enzyme Immobilization and Stabilization within Meso-Structured Onion-Like Silica for Biodiesel Production

Wednesday, October 31, 2012: 10:40 AM
407 (Convention Center )
Seung-Hyun Jun, Department of Chemical & Biological Engineering, Korea University, Seoul, South Korea, Jinwoo Lee, Department of Chemical Engineering and School of Environmental Science & Engineering, Pohang University of Science and Engineering, Pohang, South Korea, Taeghwan Hyeon, National Creative Research Center for Oxide Nanocrystalline Materials and School of Chemical and Biological Engineering, Seoul National University, Seoul, South Korea and Jungbae Kim, Chemical and Biological Engineering, Korea University, Seoul, South Korea

Highly Efficient Enzyme Immobilization and Stabilization within Meso-Structured Onion-like Silica for Biodiesel Production

Seung-Hyun Jun, Jinwoo Lee, Taeghwan Hyeon, and Jungbae Kim*

Department of Chemical and Biological Engineering, Korea University, Seoul, Korea

Advanced Functional Nanomaterials Laboratory, Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Kyungbuk 790-784, Korea

National Creative Research Initiative Center for Oxide Nanocrystalline Materials and School of Chemical Engineering, Seoul National University, Seoul 151-744, Korea

Abstract

Enzyme was immobilized and stabilized in meso-structured onion-like silica (Meso-Onion-S), which has highly-curved meso-pores of 10 nm diameter in a multi-shell structure in a form of nanoscale enzyme reactors (NERs). NERs were prepared via a two-step process of enzyme adsorption and subsequent enzyme crosslinking, which effectively prevents the leaching of crosslinked enzyme aggregates from highly-curved mesopores of Meso-Onion-S. As a result, NERs in Meso-Onion-S significantly improved the enzyme stability as well as the enzyme loading. For example, NER of lipase (NER-LP) was stable under rigorous shaking for 40 days, while the control sample of adsorbed LP (ADS-LP) with no enzyme crosslinking showed a rapid inactivation due to rigorous enzyme leaching under shaking. Stable NER-LP was successfully employed to produce biodiesels, fatty acid methyl esters, from the LP-catalyzed transesterification of soybean oil with methanol. Interestingly, the specific activity of NER-LP was 23 and 10 times higher than those of free LP and ADS-LP, respectively, revealing the importance of LP stabilization in the form of NER-LP in the presence of organic solvents.


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