268608 Nonlinear Direct Inverse Method: A Way to Combine Nonlinear Calibration with Isotherm Determination
Nonlinear direct inverse method: a way to combine nonlinear calibration with isotherm determination
of Chemical and Biomedical Engineering
Nanyang Technological University
62 Nanyang Drive, Singapore 637459
This work addresses a way to combine nonlinear calibration with isotherm determination. In this method, like classical inverse method, experimental elution profiles are compared with the results of a detailed model that accounts for nonlinearity in equilibrium, axial dispersion, and mass transfer kinetics. However, unlike classical inverse method, the calibration of detector is carried out simultaneously with isotherm determination thereby reducing cost and saving time. In this study no limitation is imposed on the linearity of the detector signal or on the overlapping of elution profiles in case of enantiomers. The method has been experimentally verified for the separation of a mixture of guaifenesin enantiomers over a wide range of operating parameters.