268574 Comparison of Oleaginous Fungal Lipid Accumulation On Different Lignocellulosic Feedstocks

Thursday, November 1, 2012: 10:10 AM
303 (Convention Center )
Zhenhua Ruan1, Mike Zanotti1, Yuan Zhong2, Chad Ducey3, Wei Liao1 and Yan Liu1, (1)Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI, (2)Biosystems and Agricultural Engineering, Michigan State Univeristy, East Lansing, MI, (3)Werks Management, LLC, Fishers, IN

The perennial grasses miscanthus, giant reed, and switchgrass, along with the agricultural residue corn stover, were evaluated for their bioconversion potential for fungal lipid accumulation. A co-hydrolysis process was adopted to convert lignocellulose into fermentable sugars. The process utilized dilute sulfuric acid pretreatment without detoxification and liquid-solid separation, directly followed by enzymatic hydrolysis. A factorial experiment in a randomized block design was employed to optimize the co-hydrolysis process. The influence of combined severity on the susceptibility of pretreated substrates by enzymatic hydrolysis was clearly discernible, showing that co-hydrolysis can be applied to lignocellulosic biomass to enhance the efficiency of sugar conversion, and reduce the amount of processing water.. The experimental results demonstrated that under the optimal conditions, corn stover had the highest sugar yield followed by switchgrass, giant reed and miscanthus. Then, an oleaginous fungus Mortierella isabellina ATCC42613 was selected and applied on hydrolysates to accumulate fungal lipids due to its capability of utilizing both C5 and C6 sugars. The cultivation using hydrolysates as the carbon source exhibited greater cell mass, and equivalent lipid production compared to synthetic medium with pure glucose and xylose. These results elucidated that combining fungal fermentation and co-hydrolysis to accumulate lipids could have potential to enhance utilization efficiency of lignocellulosic feedstocks for advanced fuels production.

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