257137 Study of the Mechanisms of Antibody Aggregation in Solution

Tuesday, October 30, 2012
Hall B (Convention Center )
Frida Ojala1, Marcus Degerman2, Thomas Budde Hansen3, Ernst Broberg Hansen3 and Bernt Nilsson2, (1)Department of Chemical Engineering, Lund University, Lund, Sweden, (2)Lund University, Lund, Sweden, (3)Novo Nordisk A/S, Bagsværd, Denmark

In order to avoid aggregation during preparative separation of antibodies, it is desirable to have thorough knowledge of the mechanistic behavior of the reaction. A study of antibody aggregation occurring in a solution has been conducted with the main aim to find a methodology to determine the mechanisms of the reaction.

The aggregation reaction is sensitive to antibody and salt concentration as well as pH and temperature. A time series of the monomer, dimer and high molecular weight product (HMWP) under varying conditions were studied to produce a time dependent model of the system. The experiments showed a significant increase in dimer concentration and a low formation rate of HMPW at low salt concentrations. At high salt concentrations however, the situation was the opposite, with higher formation rate of HMWP and low formation rate of dimers. Calibrations to different reaction models were then conducted, estimating the kinetic parameters and searching for a likely mechanism.

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See more of this Session: Poster Session in Bioseparations
See more of this Group/Topical: Separations Division