255550 ABE Fermentation In a Continuous and Closed-Circulating Fermentation System with PDMS Membrane Bioreactor

Wednesday, October 31, 2012: 1:45 PM
335 (Convention Center )
Chunyan Chen and Zeyi Xiao, chemical engineering, sichuan university, chengdu , China

Acetone-Butanol-Ethanol (ABE) fermentation by combining a PDMS membrane bioreactor and clostridium acetobutylicum was studied, and thereby a long-term continuous and closed-circulating fermentation system for ABE production has been achieved. Two rounds of experiments lasting for 274h and 300h were conducted respectively. The operation mode of the first round was fermentation intermittently coupling with pervaporation, and that of the second round was continuously coupling. The cell concentration, cumulative butanol concentration, butanol volumetric productivity and glucose consumption rate of the two cycles of fermentation were 1.59 and 1.68 gL-1, 28.03 and 61.43 gL-1, 0.11 and 0.21 gL-1h-1, 0.63 and 1.12 gL-1h-1 respectively. Except for the cell concentration, the fermentation kinetic parameters of the second cycle were almost twice those of the first cycle, which indicated the operation mode of fermentation continuously coupling with pervaporation was superior to that of intermittently coupling. Besides, at least two peak values were observed in the profiles of cell growth, glucose consumption and butanol production, which meant the microorganism revived in the PDMS membrane bioreactor system. The prolonged fermentation duration and reasonable fermentation kinetics indicated that ABE fermentation (especially for butanol production) in a continuous and closed-circulating fermentation system with PDMS membrane bioreactor was feasible and promising.


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