Synovial Fluid and Staphylococci

Monday, October 17, 2011
Exhibit Hall B (Minneapolis Convention Center)
David A. Monteiro, Stevens Institute of Technology, Hoboken, NJ

Despite improvements in our capability to design and produce implants, bacterial infection via biofilm formation remains one of the leading causes of implant failure. There is a critical need to understand more about the organisms that form biofilms and the mechanisms by which they are produced. In doing so, it is important to explore the extent of the role that environmental factors play in biofilm production. Patient samples of synovial fluid were acquired, with the idea that they would provide oligotrophic conditions while remaining relevant to orthopaedics. The extent of bacterial metabolic activity across three culture media was observed, as was the impact of nutrition on biofilm production. Confocal laser scanning microscopy allowed for characterization of the macro-structure of bacterial biofilm. Results obtained from this project will prove invaluable in the study of bacterial biofilm and it as a whole will serve as a tutorial for culturing bacteria in patient samples.

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