Effects of DNA Methylation On the Self-Assembly of a Chromatin Fiber

Tuesday, October 18, 2011
Exhibit Hall B (Minneapolis Convention Center)
Chongli Yuan, School of Chemical Engineering, Purdue University, West Lafayette, IN and Isabel Jimenez-useche, Chemical Engineering, Purdue University, West Lafayette, IN

DNA in eukaryotic cells is folded into an organized structure facilitated by a protein complex, i.e., a histone octamer. This protein-DNA complex, known as a chromatin, regulates gene expression in eukaryotic cells by limiting the accessibility of genomic DNA sequences. DNA methylation, i.e., the addition of a methyl group to a nucleotide, is known as an epigenetic mark for regulating gene expression in eukaryotic cells. Besides being a targeted site for many chromatin-binding proteins, DNA methylation has been recently postulated to directly mediate gene expression by affecting the structure of a chromatin. To verify this hypothesis and to reveal the detailed roles of DNA methylation in chromatin structure, this study examines the effects of DNA methylation on the assembling and stability of a nucleosome, i.e., the structural repeating unit of a chromatin, using various biophysical approaches. This knowledge will shed light on the mechanism of DNA packaging in eukaryotic cells.

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See more of this Session: Poster Session: Nanoscale Science and Engineering
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