Immuno-Liposome Nanoparticles for Single Cell Array

Immuno-Liposome Nanoparticles for Single Cell Array

Kwang Joo Kwak^1,2

 

¹NSF Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Device (NSEC-CANPBD)

²Department of Chemical and Biomolecular Engineering

The Ohio State University, Columbus, Ohio 43210

*Contact author- E-mail: kwak.63@osu.edu

Recent advances in biomimetic lipid membranes provide opportunities to develop applications such as biosensing, gene/drug delivery, and cellular recognition. My research interests are focused on tethered immuno-liposome nanoparticles (t-ILNs) that can be conjugated on a planar surface through a self-assembled monolayer of a thiolipid and modified with cell adhesion molecules and therapeutic targeting antibodies. The multi-antibody conjugated t-ILNs have been investigated to be able to isolate extremely rare cells such as cancer stem cells from the whole human blood. The t-ILNs array based on a centrifugal force has been demonstrated to improve sensitivity and specificity of stem cell isolation by increasing the focal adhesion between cell and the ILN surface and preventing non-specific binding on the interfaces. Novel t-ILN array based cell isolation has higher potential for many other important biomedical applications such as prognosis of cancer patients and monitoring targeted therapy.