Enzyme Stabilized Systems Using Organic/Inorganic Hybrid Materials

In this study, we report highly stable enzyme immobilized systems - using nano/micro-sized hybrid materials.  Hydrolase enzyme, esterase from Rhyzopus oryzae, has been studied as a model enzyme in aqueous condition for increasing storage and recycling stability.  This esterase enzyme has been subjected to be immobilized in a form of entrapment and/or coating on the surfaces by using calcium phosphate nanoparticles or organic-inorganic hybrid microspheres prepared as porous support materials for good accessibility of substrate.  These nano/micro-sized hybrid materials were successfully characterized by using Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), Transmission electron microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDX).  As results, the nano/micro-sized hybrid materials were found to be efficient enzyme immobilizing carriers.  The immobilized esterase composite systems have shown the good storage stability retained in terms of its enzyme activity preserved at the repeated usages for more than ten times.  Therefore, it is strongly anticipated that this enhanced strong stability obtained from these enzyme hybrid systems will boost the employment of these systems in various fields of enzyme related applications, such as bioprocessing, bioremediation, biosensing, or enzyme reactors.