Clustered-Charge Anion Exchange Adsorbents: Nucleic Acid Affinity and Single-Molecule Characterization

Tuesday, November 9, 2010: 9:24 AM
255 B Room (Salt Palace Convention Center)
Wen-hsiang Chen, Mohan Vivekanandan Poongavanam and Richard C. Willson, University of Houston, Houston, TX

Ion-exchange chromatography is widely used for purification and separation of biomolecules. Traditionally, charged ligands are introduced into ion-exchange matrices by random chemical processes, producing a heterogeneous charge distribution. In previous work, we demonstrated improved selectivity and affinity of adsorbents in which charges are introduced in a clustered, rather than random fashion.

The current work establishes the enhanced DNA adsorption affinity and capacity of clustered pentaargininamide vs. equal-charge argininamide adsorbents. The effects of ligand density are explored, as well as mechanisms of maximizing DNA selectivity over RNA. Early results on the single-molecule monitoring of biomolecule adsorption in realistic agarose adsorbents also will be presented.

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See more of this Session: Bioseparations and Downstream Processing
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division