Hydrophobic Interaction Chromatography of Proteins Using Environment Responsive Membranes

Monday, November 8, 2010: 1:45 PM
253 B Room (Salt Palace Convention Center)
Raja Ghosh, Xiaojiao Shang and Deqiang Yu, Department of Chemical Engineering, McMaster University, Hamilton, ON, Canada

Environment responsive membranes are being examined for a wide range of applications such as bioseparations and controlled release of drugs. In this presentation, we discuss the use of environment-responsive, hydrophilized microporous membranes for hydrophobic interaction chromatography of proteins. These membranes can be made to switch between hydrophobic and hydrophilic states in a reversible manner by controlling salt concentration. In contrast, media used for conventional hydrophobic interaction chromatography (HIC) remain hydrophobic through a separation process. These environment responsive membranes therefore give better resolutions than conventional HIC media. Proteins with very subtle hydrophobicity differences can be separated. Some examples of these are discussed.

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See more of this Session: Membranes for Bioseparations
See more of this Group/Topical: Separations Division