Biohydrogenation From Biomass Sugar Mediated by in Vitro Synthetic Enzymatic Pathways

Wednesday, November 10, 2010: 3:15 PM
255 D Room (Salt Palace Convention Center)
Percival Zhang and Yiran Wang, Virginia Polytechnic Institute and State University, Blacksburg, VA

Biohydrogenation catalyzed by enzymes is becoming more accepted, especially for synthesis of chiral compounds. The pairs of substrate/enzyme for NAD(P)H generation include alcohol/alcohol dehydrogenase, formate/formate dehydrogenase, glucose/glucose dehydrogenase, glucose-6-phosphate (G6P) /G6P dehydrogenase, dihydrogen/hydrogenase, and phosphite/phosphite dehydrogenase. Here we demonstrate high-yield generation of NAD(P)H from a renewable biomass sugar cellobiose through an in vitro synthetic enzymatic pathway (SyPaB) consisting of 12 purified enzymes and coenzymes. The NADPH yield was as high as 11.4 mol NADPH per cellobiose (i.e., 95% of theoretical yield 12 NADPH per glucose unit) in a batch reaction. Consolidation of endothermic reactions and exothermic reactions in one pot results in the biohydrogenation reaction between xylose and cellobiose with an amazingly high energy-retaining efficiency of 99.6%. The high-yield and low-cost production of polyols from the fermentation-toxic biomass hydrolysate by SyPaB might be important to produce hydrocarbons by aqueous phase reforming.

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See more of this Session: Advances in Biocatalysis and Biosynthesis II
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division