Advances in Proteomics: New Technologies

Wednesday, November 11, 2009: 8:30 AM
Canal D (Gaylord Opryland Hotel)

Description:
Studies of protein expression patterns play a vital role in understanding the complex responses of cells, tissues, and organisms to stimuli or mutations. While recent developments have allowed these patterns to be investigated at an unprecedented level of detail, further advances are needed in order to fully illuminate the interplay among the many factors governing cellular response. Specifically, new technologies are needed that provide quantitative information with high sensitivity and throughput. This session will focus on the development of such proteomic technologies and their applications. Of particular interest are papers describing advances in electrophoretic protein separations, novel means of detecting and quantifying proteins, methods of analyzing specific protein classes, mass spectroscopic methods, and other related technologies. Papers are also sought that present research on the proteomic analysis of post-translational modifications.


Sponsor:
Topical 3: 2009 Annual Meeting of the American Electrophoresis Society (AES)


Chair:
Tom Berkelman
Email: Thomas_Berkelman@bio-rad.com

Co-Chair:
Phil Beckett
Email: phil.beckett@ge.com


8:30 AM
(342a) Comparison of High Abundance Protein Depletion Techniques
Aran Paulus, Tim Wehr, Chengjun Sun, Lei Li, Katrina Academia, Steve Freeby, Ning Liu, John Walker and Chris Sutton

8:50 AM
(342b) Fluorescent Zdye Platform for Differential Covalent Labeling of Proteins for 2D and 1D Gel Analysis
Edward Dratz, Paul Grieco, Mary Cloninger, Kristian Schlick, Duane Mooney, Ting Liu, Matt Shipman, Scott Laffoon, Jared Bowden, Ben Reeves, Kevin Spicka, Hannes Helmboldt, Ben Skerik-Borg, Brian Richards, Casey Smith and Rand Swanson

9:30 AM
(342d) Optimization of 2D Gel Transblotting of Complex Protein Mixtures
Nancy Kendrick, Jon J. Johansen and Matthew Hoelter

10:10 AM
(342f) A De Novo Approach for Untargeted Post-Translational Modification Prediction Using Tandem Mass Spectrometry and Integer Linear Optimization
Richard Baliban, Peter A. DiMaggio Jr., Nicolas Young, Benjamin A. Garcia and Christodoulos A. Floudas

10:30 AM
(342g) Detecting Low Abundance Proteins in Skinned Cardiac Myofibrils
Rylie Siu, Qian Xu, Shannamar Dewey, Susan Nguyen and Aldrin V. Gomes