2D HSQC NMR Analysis and Application to Hot-Water Wood Extract Hydrolysate Characterization

Hot-water extraction has been evolved into a necessary biorefinery step that enables the incremental conversion of lignocellulosic materials to chemicals, materials and energy. The extraction liquor and subsequent hydrolysate are a complex mixture of organic and inorganic substances. 5-carbon and 6-carbon sugars and their derivatives make up the bulk of the mixture. Developing a reliable technique to quantify the mixture has become the bottleneck of the hot-water extraction process development. In this study, we present the development of the 2D HSQC NMR characterization of the complex mixture. It can economically produce quantifications for the monomoeric and some of the oligomeric sugars.