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Studies on the Decontamination of Surfaces Exposed to Cytotoxic Drugs

Marek Kuzma1, Jaroslav Cerveny2, Petr Kacer3, Jiri Svrcek3, Kamila Syslova3, Libor Cerveny3, and Libor Panek4. (1) Laboratory of Molecular Stracture Characterization, Institute of Microbiology, Videnska 1083, Prague, 142 20, Czech Republic, (2) Laboratory of Molecular Structure Characterization, Institute of Microbiology, Videnska 1083, Prague, 142 20, Czech Republic, (3) Department of Organic Technology, Institute of Chemical Technology Prague, Technicka 5, Prague, 166 28, Czech Republic, (4) Block a.s., Stulikova 1392, Prague, 198 00, Czech Republic

Vapor phase hydrogen peroxide (VPHP) represents a new technology, which was widely proved as an appropriate method for decontamination of rooms. The attacks on many cities revealed the difficulty of decontaminating buildings that had been exposed to biological weapons. The well-known effects of mold on allergies and asthma are also creating demand for improved building air quality. VPHP has become the method of choice for many bio-decontamination requirements in the pharmaceutical, biomedical and healthcare sectors because it is reliable, rapid, leaves no residues and may be validated, yet there is considerable misunderstanding about how it works and the physical chemistry of the process. VPHP is used to treat pharmaceutical manufacturing clean rooms and laboratory toxicology rooms. There is rising interest in application of VPHP for decontamination of chemical warfare and biologically active compounds. But there is only limited information about influence of VPHP on chemical substances. The aim of this study was to examine the removal and deactivation of cytotoxic contamination from surfaces. It was developed appropriate experimental equipment for testing influence of VPHP on biologically active substances. Using mass spectrometry (GC/MS, LC/MS) and NMR were studied decomposition products of cytotoxic drugs. Highly sensitive and precise method combining LC with ESI-MS/MS or CG/MS was developed and used for monitoring of kinetics of decontamination of basic cytotoxic compounds used in medicinal practice.

Acknowledgements

The authors thank the Ministry of Industry and Trade of the Czech Republic (Grant No FT-TA 4/071), IMIC institutional research concept (AVOZ50200510), and Ministry of Education of Czech Republic (Grant CEZ: MSM 223 100001) for financial support.