- 12:48 PM
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Cooperativity Mechanisms and Hybridization Free-Energy Landscapes of Model Oligonucleotides In Solution

Juan C. Araque1, Athanassios Z. Panagiotopoulos2, and Marc A. Robert1. (1) Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main St. MS-362, Houston, TX 77025, (2) Chemical Engineering, Princeton University, Princeton, NJ 08540

A coarse-grained lattice model of oligonucleotides is proposed to study the role of cooperativity in the microscopic pathways and mechanisms of DNA hybridization in solution; the extent to which this transition conforms to two-state thermodynamics is also analyzed. The thermodynamic behavior of this model have been extensively investigated and shown to correspond, qualitatively, to that observed in experiment. Equilibrium populations of single- and double-stranded states, and their associated free-energy landscapes, are calculated by Monte Carlo simulations with configurational bias and parallel tempering. The two-state nature of the transition is found to exhibit strong sequence dependence. Two general scenarios are found depending on the characteristics of the free-energy surface, in which hybridization pathways are shown to evolve through a complex network of stationary points, i.e., local minima and transition states. Remarkably, although hybridization of model oligonucleotides in solution always exhibits two-state thermodynamic signatures, its reaction pathways evolve, to a varying degree, through non-two-state mechanisms that involve stable and metastable intermediates.