We hypothesize that the sepsis complications in burn patients occur due to the improper activation of neutrophils ultimately causing multiple organ failure. We study their migration behavior using microfluidic tools capable of performing fast switching between different chemokines in the gradient chamber (within ~ 5 seconds). An advanced switching gradient device incorporating microstructured valves has been developed that can be used to directly isolate primary neutrophils from just a drop (<10µL) of whole blood as well as provides a robust platform to perform chemotaxis assays in the competing environment of different chemokines. A drop of blood obtained from finger prick mixed with heparin solution is loaded in the flow chamber individually pre-coated with specific cell adhesion molecules (in this case P-selectin or E-selectin) that facilitate cell adhesion and capturing. We observe low affinity binding and rolling action of leukocytes in the absence of a stimulus while the rolling action turned into firm adhesion inside chemokine-pretreated channels. Observations from neutrophil capture characterization suggest that the P-selectin and E-selectin substrates demonstrate optimum capture efficiencies of 30-40 cells/mm2 at concentrations 25µg/mL and 50µg/mL respectively. We also performed migration experiments on these substrates under individual gradients of chemoattractants fMLP and IL-8 and the observed average velocities indicate comparable migration patterns. Similar experiments will be performed (both pre-burn and post-burn) in an overlapping environment of different chemokines (IL8, C5a, GRO-α, fMLP and LTB4) and the migration behaviors will be compared. This study could open the possibility of using simplified and inexpensive point-of-care diagnostic methods based on microfluidic devices for predicting clinical outcome in burn patients.