Thursday, November 8, 2007: 3:30 PM-6:00 PM
Room 250 B (Salt Palace Convention Center)

Bioengineering (15c)

#645 - Quantitative Metabolic Phenotyping in Plant and Animal Systems (15C06)
Metabolic flux analysis (MFA) and metabolite profiling are powerful platforms for studying biochemical reaction networks. An emerging area of interest is in the application of these approaches to address fundamental physiological questions in higher organisms, especially plant and animal systems of specific agricultural or clinical significance. In this session, we explore the extension of metabolic phenoptying to quantitative physiological studies. Applications to mammalian tissue/cell cultures, plant cell/embryo systems and whole plants/animals are of primary interest. Integrative modeling studies based on fluxomic and metabolomic data sets are also of interest.
Chair:Jamey D. Young
CoChair:Doug K. Allen
3:30 PMNovel Measurements For Flux Analysis Applied To Plant Oilseeds
Doug K. Allen, John B. Ohlrogge, Yair Shachar-Hill
3:50 PMMetabolic Flux Analysis on Soybean Isolines with Different Seed Compositions
Vidya V. Iyer, Matthew E. Studham, Mark E. Westgate, Julie A. Dickerson, Eve S. Wurtele, Jacqueline V. Shanks
4:10 PMA Nonstationary 13C Labeling Approach For Metabolic Flux Analysis In A Photoautotrophic System
Avantika A. Shastri, Jamey D. Young, Gregory N. Stephanopoulos, John A. Morgan
4:30 PMTime-Series Metabolomic Analysis For Identifying Metabolic Engineering Targets In Plant System
Harin H. Kanani, Maria I. Klapa
4:50 PMBreak
5:00 PMHigh Resolution Flux Estimation of Gluconeogenesis Using A Novel [U-13C,2H8]Glycerol Tracer in Mice
Maciek R. Antoniewicz, Jose O. Aleman, Joanne Kelleher, Gregory Stephanopoulos
5:20 PMApplication of Metabolic Flux Analysis to Identify the Mechanisms of Free Fatty Acid Toxicity to Human Hepatoma Cell Line
Shireesh Srivastava, Christina Chan
5:40 PMFlux Profile of Time-Dependent Metabolic Changes of De Novo Adipocyte Formation
Yaguang Si, Jeongah Yoon, Kyongbum Lee

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