Development of approaches to separate protein isoforms is propelled by the central role that these macromolecules have been found to have in the pathological states of disease and mutations. Protein isoforms are protein molecules that vary from each other due to differences in a small number of amino acid residues. Since isoforms are alike in their make-up, they often have similar physical characteristics that make them very difficult to identify and separate. The proposed approach aims to perform separation of protein isoforms under conditions where separation of close mobility molecules can be made extremely resolute. Such conditions will provide an environment in which differences in electrophoretic mobility and/or adsorption kinetics become more discernable and exploitable.