Arg-Gly-Asp (RGD) has been widely utilized to increase cell adhesion to three-dimensional scaffolds for tissue engineering. However, cell seeding on these scaffolds has only been carried out statically, which yields low cell seeding efficiencies and poor cell spatial distribution. Scaffold seeding is important in the production of functional tissue engineering constructs; therefore, more efficient seeding techniques are needed. We have characterized, for the first time, the seeding of rat mesenchymal stem cells on RGD-modified poly(L-lactic acid) (PLLA) foams using oscillatory flow perfusion. PLLA foams were soaked in an acetone-water mixture, and then incubated in a solution of poly (L-lysine) (polyK). RGDC peptides were linked to the polyK through a disulfide bond, using SPDP as the linker. RGDC surface concentration was varied by changing the polyK incubation concentration. Inoculated cell suspensions were placed on top of modified scaffolds accommodated in the chambers of a flow perfusion bioreactor and oscillatory flow was applied at 0.15 ml/min for 2h. Cells were allowed to condition for 2h without flow; unidirectional flow was incorporated at 0.15 ml/min and increased after 2h to different flow rates. These flow rates included 0.15, 0.5 and 1.0 ml/min. The incorporation of RGD on the PLLA foams improves scaffold cellularity in a dose-dependent manner under oscillatory flow perfusion seeding. When compared to static seeding, oscillatory flow perfusion is the most efficient seeding technique. Cell detachment studies show that cell adhesion is dependent on the applied flow rate, and that cell attachment is strengthened at higher levels of RGD modification.