Roller bottles are prevalent throughout the cell culture industry because they are mechanically simple to operate, and the culture process is easily scalable since several are able to run simultaneously on a bank of roller apparatuses. Roller bottles are deficient, however, in that they only operate as batch or fed-batch systems, preventing them from achieving high cell densities and antibody secretion rates that are possible in continuous perfusion systems. The concept of a continuous perfusion self-feeding roller bottle, which is mechanically simplistic and works with existing roller apparatuses, is presented here. A conventional roller bottle is partitioned into two chambers; one chamber contains the fresh culture media reservoir, and the other contains the cell culture chamber. A spiroid of tubing inside the fresh media reservoir acts as a pump when the bottle rotates on its horizontal axis, continuously delivering fresh media through an opening in the partition to the cell culture chamber. The modified bottle proved capable of maintaining steady-state cell densities of a hybridoma cell line over the 10 day period tested, although at lower densities than reached during batch operation due to the continuous volume dilution. Steady-state density proved to be controllable by adjusting the perfusion rate which changes with the rotation rate of the bottle. Specific antibody production rate is as much as 3.7 times the rate in conventional roller bottles operating with intermittent batch feeding. High oxygen levels in the perfused media, a result of the spiroidal pumping liquid delivery system, offers the potential for higher operating volumes and cell mass than in conventional roller bottles.