This work presents the results of a study of cleaning medical equipment with compressed CO2. The implantation of contaminated biomaterials may result in infections, severe pain, and rejection of the implant by the recipient. Industry has been seeking for alternatives to the water and to toxic or hazardous chemicals in cleaning operations. Compressed CO2 has been identified as a promising alternative solvent for cleaning applications, because of its unique chemical and physical properties and because of its potential as a technology platform for sterilization. This work evaluated the efficacy of compressed CO2 and compressed CO2-based mixtures in the removal of oily contaminants and proteins from metal surfaces commonly used in surgical implants. Metal surfaces were contaminated with either a bio-lubricant oil or bovine serum albumin (BSA), a model protein, in order to simulate contaminations resulted from manufacturing and hospital operations. The oily metal surfaces were treated with compressed CO2 alone while the BSA contaminated surfaces were treated with mixtures of compressed CO2, surfactant LS-54 and water. Removal of contaminants was analyzed with a gas chromatograph (for oil) and UV/VIS spectrometer (for both oil and BSA). Compressed CO2 at relatively low temperatures and moderate pressures can be used to remove oil and BSA from metal surfaces, proving that CO2-based cleaning processes may be feasible for removing these contaminants from medical devices.