Thursday, November 8, 2007 - 2:20 PM

Nanoporous Biodegradable Systems for Cellular Delivery of Embryonic Stem Cells

Xulang Zhang1, Hongyan He2, Yubin Xie3, Yuan Yuan1, and L. James Lee1. (1) The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, (2) Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 WEST 19TH AVE, Columbus, OH 43210, (3) University of Albany, 255 Fuller Road, Albany, WA 12203

Cell-based delivery devices holds great promise for applications requiring site-specific and sustainable drug delivery of cell-synthesized molecules. On the other hand, embryonic stem (ES) cell therapies have been proposed for regenerative medicine and tissue replacement after injury or disease. An integration of ES cells within immunoprotective and biodegradable devices will provide great potential for novel delivery platforms. In this study, nanoporous microcapsules and chambers based on biodegradable Alginate/polylysine and poly (lactide-co-glycolide acid) (PLGA) are developed for immunoisolation of embryonic stem cells and control release of therapeutic products for comparison. First, secreted alkaline phisphatase gene was tansfected into the mES cells using amaxa transfection system and then transfected mES cells were trapped into microcapsules and PLGA chambers. Studies described herein also include the interactions of biodegradable-based substrates with mouse ES cells in terms of viability, proliferation, and drug delivery efficiency. At the same time, immunoisolation effect of nanoporous membrane was analyzed by incubation with IgG molecule In addition to reporter gene of secreted alkaline phosphate, mouse ES cells can also be genetically engineered to secrete therapeutic drugs, such as dopamine and insulin, which can be integrated in nanoporous biodegrade-based devices for the treatment of neurological and endocrinal diseases.