The reaction could be described by a Ping Pong Bi Bi mechanism with alcohol inhibition. No significant differences were found between the parameters found on micro scale and those found on bench scale. Mass limitation does not appear to play an important role in the reaction rate. Rather, the reaction is kinetically limited. As the kinetics on both scales are similar, parameters found on either one can be used in processing on the other scale.
Enzyme kinetic parameters can be determined on a micro scale, with very low consumption of reagents and catalyst, and then be applied to bench scale. This can reduce the cost of optimizing enzyme processes by downscaling.