Thursday, November 8, 2007 - 10:35 AM
520f

Optimization of Medium Constituents for the Production of L-Glutamic Acid by Brevibacterium Roseum Using Response Surface Methodology

Murali Yugandhar Nikku1, Sri Rami Reddy Donthireddy1, Asha Immanuel Raju Chaduvula1, Kiran Babu Uppuluri2, Sarat Babu Imandi3, Kiran Kumar Nalla4, and V. R. Ravi Kumar Dasari3. (1) Chemical Engineering Department, Andhra University, Andhra University, Visakhapatnam - 530 003, AP, INDIA, Visakhapatnam, 530003, India, (2) Nagarjuna University, Bapatla Engineering College, Bapatla, India, (3) Chemical Engg, Center for Biotechnology, Room No: 109, Andhra University, Visakhapatnam, 530003, India, (4) Chemical Engg, Center for Energy Engg, Room No: 109, Andhra University, Visakhapatnam, 530003, India

Abstract

Statistical experimental design was used to optimize the conditions viz. Ethanol, Ammonium phosphate and Biotin concentration of L-Glutamic acid from Brevibacterium roseum NCIM 2238 by submerged fermentation. Maximum L-Glutamic acid concentration of 53.01621 g/l was obtained. The optimum conditions were found to be ethanol concentration of 5.3646 %v/v, ammonium phosphate of 0.7081 %w/v and biotin concentration of 1.6735 µg/ml. Glutamic acid production was also effectively promoted by optimizing the medium composition using Response Surface Methodology (RSM). Basically, this optimization process involves three major steps: performing the statistically designed experiments, estimating the coefficients in the mathematical model, and predicting the response and checking the adequacy of the model. Thus, by using SmF process with Brevibacterium roseum, the Central Composite Design (CCD) was found to be the most favorable strategy for investigating with respect to L-Glutamic acid production.


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