Thursday, November 8, 2007 - 1:24 PM

Micropatterned Fluid Lipid Bilayers Created Using A Continuous Flow Microspotter For Multi-Analyte Assays

Kathryn A. Smith1, Bruce K. Gale2, and John C. Conboy1. (1) Department of Chemistry, University of Utah, 315 S. 1400 E. RM. 2020, Salt Lake City, UT 84112, (2) Department of Mechanical Engineering, University of Utah, 50 S. Central Campus Drive, Salt Lake City, UT 84112

There is an increasing interest in the development of microarray based assays for high-throughput analysis and detection of biomolecules such as DNA and proteins. We have developed a new method for creating multianalyte planar supported lipid bilayer (PSLB) arrays using a 3D continuous flow microfluidic system (CFM). PSLBs are an attractive platform for performing biological assays due to their high resistance to nonspecific biomolecule adsorption and non-fouling nature. PSLBs also serve as good models of biological membranes, allowing a diverse assortment of biological and chemical interactions to be explored in a controlled manner in vitro. The CFM allows for an addressable microchannel to be formed, in which an array of fluid bilayers can be patterned onto a glass substrate. The system's capability of performing multianalyte assays has potential applications in many fields such as biosensing, drug discovery, proteomics and clinical diagnostics.