Tuesday, November 14, 2006: 3:15 PM-5:45 PM
Yosemite A (Hilton San Francisco)
#265 - Advances in Proteomics: New Technologies II (T3002)
Systematic studies of gene expression patterns play a vital role in understanding the complex interactions associated with the global response of cells, tissues, and organisms to stimuli or mutations. While recent developments have allowed these patterns to be investigated at an unprecedented level of detail, further advances are needed in order to fully illuminate the interplay among the many factors governing cellular response. Specifically, new technologies are needed with the capability of providing quantitative information with greatly enhanced levels of sensitivity and throughput. This session will focus on the development of these proteomic technologies and their application to biotechnology. Of particular interest are papers describing advances in gel-free protein separations, novel protein stains, methods of analyzing membrane proteins, mass spectroscopic methods for resolving mixtures of peptides, high-throughput antibody creation and optimization, the development of protein-detecting microarrays, and other related technologies. Papers are also sought that present research on the application of proteomics to the study of bacterial, animal, and plant cell cultures, and especially proteomic analysis of post-translational modifications.
Chair:Feng Wang
3:15 PMPhosphopeptide Analysis Using a Planar Electrochromatography/ Thin-Layer Chromatography Separations Platform Coupled to Orthogonal Maldi-Tof Mass Spectrometry
Wayne F. Patton, Venkateswarlu Panchagnulu, Alvydas Mikulskis, Ira S. Krull
3:40 PMPressurized Planar Electrochromatography: a New Tool for Proteomics?
David Nurok, Allyson L. Novotny, Robert E. Santini, Randall W. Replogle, Gregory L. Hawkins
4:05 PMProteomic Analysis of Immunogenic Proteins in Xenogeneic Heart Valve Bioscaffolds
Leigh G. Griffiths, E. Christopher Orton, Kenneth F. Reardon
4:30 PMA Closer Look at E. Coli-Derived 6-Deoxyerythronolide B Biosynthesis
Brett A. Boghigian, Yong Wang, Blaine A. Pfeifer
4:55 PMProteome Analysis of Pseudomonas Putida during Biodegradation of High Concentration of Benzoate:Activation of the Meta Pathway and Physiological Responses
Kai Chee Loh, Bin Cao
5:20 PM2d Gel Phosphoprotein Western Blotting
Nancy Kendrick, Jon Johansen, Matthew Hoelter

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