Byoung Chan Kim1, Jin Hyung Lee1, Jungbae Kim2, and Man Bock Gu3. (1) Advanced Environmental Monitoring Research Center (ADEMRC), Gwangju Institute of Science and Technology (GIST), 1, Oryoung-dong, Puk-Gu, Gwangju, South Korea, (2) Department of Chemical and Biological Engineering, Korea University, Seongbuk-Gu Anam-Dong, Seoul, 136-701, South Korea, (3) Colleage of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Sungbuk-gu, Seoul, South Korea
The use of nanostructured materials is an intriguing approach for developing composite of enzyme-nanomaterials since these materials can provide a large surface area, which can lead to high volumetric enzyme activity. Recent advances in enzyme aggregation protocols via a cross-linking method are promising for the development of highly stable and active composite of biocatalytic-nanomaterials. Significant enhancement of enzyme stabilization was realized by introducing cross-linked enzyme aggregates (CLEAs) via glutaraldehyde mediated coupling with nanofibers. The enzyme stability of enzyme aggregated nanofibers was greatly enhanced with essentially no measurable loss of activity over a month or several days of observation under rigorous shaking condition. The nanofibers coated with proteases and esterases aggregation, repectively were implemented for application in biodegradation. This approach offers a useful biocatalytic system with potential applications in biosensor, bioelectrochemistry, bioremediation and bioconversion.