- 3:55 PM
612c

Stem Cell Microarrays: Investigation of Factors That Influence Neural Stem Cell Fate

Randoph S. Ashton1, Joseph Peltier2, Christopher Fasano3, Analeah O'Neil2, Sally Temple3, David V. Schaffer2, and Ravi S. Kane1. (1) Chemical and Biological Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, (2) Chemical Engineering, University of California, Berkeley, 278 Stanley Hall, Berkeley, CA 94720, (3) Center for Neuropharmacology and Neuroscience, Albany Medical College, Albany, NY 12208

We have developed a high throughput microarray-based approach for gene function screens in stem cells. Our microarrays are generated using soft lithographic microfabrication techniques; the method can be used for both loss and gain of function screens, and is compatible with both adult and embryonic stem cells. The method allows us to assay in parallel various cell functions including signaling, differentiation, and proliferation. To demonstrate the utility of this approach, we have screened cDNA libraries for factors that affect adult neural stem cell function. Our method would facilitate genome-scale gene function studies in a variety of mammalian cells, as well as the identification of drug targets and the generation of therapies for neurodegenerative diseases.