The pump was demonstrated to be effective and robust for micro-fluid injection analysis (µ-FIA) as a fluid-driving unit. The micro FIA system consists of a monolithic micro-pump on a glass slide (2.5 cm 7.5 cm), a micro-injector and a micro-sensor (2.5 cm 1.5 cm). An electro-active neurotransmitter, dopamine, was used as the test sample. Chronoamperometric measurement of dopamine oxidation on microchip sensor verified the applicability of the monolithic pump. In addition, we also demonstrated that, by changing the polarity of the electrodes, the same electric potential was harnessed to generate a stable electrospray (ES) electrostatically directly from the porous emitter without the assistance of an upstream micropump. Characterization of the electrospray process by optical and electrical monitoring was carried out. Four typical spray modes were obtained depending strongly upon the coupling between the micropump and electrospray. Therefore, a fully integrated EO/ES coupling system was realized.
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