Tuesday, November 6, 2007: 12:30 PM-3:00 PM
Room 355 E (Salt Palace Convention Center)

Topical 3: 2007 Annual Meeting of the American Electrophoreses Society (AES) (T3)

#215 - Advances in Proteomics: New Technologies II (T3005)
Systematic studies of gene expression patterns play a vital role in understanding the complex interactions associated with the global response of cells, tissues, and organisms to stimuli or mutations. While recent developments have allowed these patterns to be investigated at an unprecedented level of detail, further advances are needed in order to fully illuminate the interplay among the many factors governing cellular response. Specifically, new technologies are needed with the capability of providing quantitative information with greatly enhanced levels of sensitivity and throughput. This session will focus on the development of these proteomic technologies and their application to biotechnology. Of particular interest are papers describing advances in gel-free protein separations, novel protein stains, methods of analyzing membrane proteins, mass spectroscopic methods for resolving mixtures of peptides, high-throughput antibody creation and optimization, the development of protein –detecting microarrays, and other related technologies. Papers are also sought that present research on the application of proteomics to the study of bacterial, animal, and plant cell cultures, an especially proteomic analysis of post-translational modifications.
Chair:Yue Ge
CoChair:Timothy Haystead
12:30 PM215aQuick, Easy, Reproducible Protein Isoelectric Fractionation Using Protein Forest digital ProteomechipTM Technology
Aaron Sin, James Dasch, William Skea, Russell Garlick
12:48 PM215bAn Orthogonal Affinity Capture and Detection Strategy for Identifying and Characterizing Phosphoproteins Directly from Sds-Polyacrylamide Gels: Inorganic Chemistry to the Rescue!
Alvydas Mikulskis, Linan Song, Alla Bogdanova, Hao Xie, Eva Golenko, Yang Wang, Wayne F. Patton
1:06 PM215cInstrumentation and Protocols for Optimized Separation of Peptides by Pressurized Planar Electrochromatography, with Subsequent Direct Interrogation of Analytes by Tandem Mass Spectrometry
Wayne F. Patton, David Bourdon, Peter Jackson, Mei Wang, Yang Wang, Linan Song, Bob Krug, Steven Liebold
1:24 PM215dA Hybrid Methodology For Peptide Identification Via Mixed-Integer Linear Optimization, Local Alignment Database Search And Tandem Mass Spectrometry
Peter A. DiMaggio, Christodoulos A. Floudas
1:42 PM215eIntegrated Top-Down And Bottom-Up Strategy For Characterizing Intact Proteins And Their Modifications
Ljiljana Pasa-Tolic
2:00 PM215f15N Metabolic Labeling And Tandem Mass Spectrometry: Tools For The Proteome Characterization Of The Facultative Psychrophile pedobacter Cryoconitis Via Ortholog Searching And de Novo Sequencing
Ana G. Pereira-Medrano, Rosa Margesin, Phillip C. Wright
2:18 PM215gIdentification Of Phosphotyrosine-Containing Proteins By Immunoprecipitation And Mass Spectrometry
Nancy Kendrick, Jon J Johansen, Matthew Hoelter
2:36 PM215hFabrication Of Cell-Free Expressed Protein Libraries For Proteomics: The Use Of T-RNA Engineering, Photocleavable Linkers And Multiplexed Protein Production On Beads
Mark J. Lim, Sadanand Gite, Ziying Liu, Kenneth J. Rothschild

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