The aim of this study is to further the understanding of pulmonary surfactant adsorption and the resultant film mechanics. Pulmonary surfactant is a complex mixture of lipids and proteins that forms a film within the terminal airspaces and prevents the airspaces from collapsing during exhalation. The proper function of pulmonary surfactant is essential for normal respiration. Premature infants that lack sufficient surfactant suffer from Respiratory Distress Syndrome, the treatment for which is the instillation of an exogenous surfactant replacement. The exogenous surfactant is generally pulmonary surfactant obtained from bovine or porcine sources. Dysfunction of the surfactant layer occurs in Acute Respiratory Distress Syndrome. Although surfactant dysfunction is a consequence rather than a cause of this syndrome, ongoing clinical trials are investigating the efficacy of exogenous surfactant replacement therapy. However, surfactant therapy is expensive and the supply is limited. There is a clear need for a mechanistic understanding of the function of pulmonary surfactant and the components thereof in the context of respiratory cycling to aid in the development of inexpensive, readily available synthetic surfactant replacements. To function effectively, surfactant must adsorb quickly and remain at the interface during respiratory cycling, forming films of near zero surface tension during exhalation. Preliminary data on a simple model system of one component of pulmonary surfactant have shown that the pathway of film formation has an effect on the properties of the adsorbed film. This study has tested the following hypotheses. First, that factors that affect transport but do not affect the microstructure of the surface-active aggregate do not affect the mechanics of the resultant film. Second, factors that affect the microstructure of the surface-active aggregate affect the mechanics of the resultant film. The primary goal of this study is to emphasize that the dynamics of surfactant adsorption and the mechanical response of the resultant film are intrinsically coupled.