Chee Guan Koh1, Xulang Zhang2, Bo Yu1, Xiaojun Yang3, Yan Jin2, Jingjiao Guan2, Zhengzheng Fei1, Robert J. Lee3, and L. James Lee4. (1) Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, (2) NSF Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, (3) College of Pharmacy, The Ohio State University, 542 Parks Hall, 500 West 12th Avenue, Columbus, OH 43210, (4) Chemical and Biomolecular Engineering, The Ohio State University, 125A Koffolt, 140 West 19th, Columbus, OH 43210
In recent years, the delivery of therapeutic genes by non-viral vectors (polyplexes, lipoplexes, or lipopolyplexes) has gained significant interest. The systemic application of non-viral vectors has been limited by rapid clearance from the bloodstream following intravenous injection. Non-viral vectors that contained surface shielding and targeting ligands such as poly(ethylene glycol) (PEG) and transferrin or folate, respectively, have been shown to improve circulation time and specificity.
In this study, we have developed multifunctional lipopolyplex containing antisense oligonucleotide for targeting Bcl-2 down regulation. Using K-562 human erythroleukemia cells as a model cell line, cell viability, gene transfection efficiency, and Bcl-2 down regulation are examined. We have characterized the lipopolyplex size and surface charge by dynamic light scattering and zeta potential measurement. In addition, a novel microfluidic approach will be used to synthesis the lipopolyplexes and compared with conventional bulk mixing method.