Hyaluronic acid and chitosan interact to create a polyelectrolytic complex of fibers within an aqueous environment. The resulting hydrogel exhibits viscoelastic properties and is an excellent matrix for cell adhesion and growth. The complex formation is dependent on the blending of the dry materials prior to hydration. Scanning electron microscopy is used to visualize the blending of the materials. Once the polyelectrolytic complex has formed, the spatial arrangement of the biopolymers must be evaluated. Colloidal gold has been conjugated to chitosan for imaging using transmission electron microscopy. Texas red has been conjugated to the hyaluronic acid to visualization with the complex fibers. The biopolymer molecular weight, the extent of chitosan deacetylation, the proportion of hyalruonan to chitosan, and the blending of materials all contribute to the final morphology of the hydrated material.