Ethoxylated nonionic surfactants are used in many industrial applications, which involve their adsorption on various interfaces. Fundamental understanding and quantification of the adsorption behavior is therefore important. In the present study, adsorption behavior of pure ethoxylated nonionic surfactants on cotton and polyester surface in hydrophobic environment was quantified. The role of ethylene oxide group variation in surfactant-polymer interaction was established using pure surfactant with same alkyl chain length but varying ethoxylate chain lengths. It was observed that surfactant with more ethylene oxide groups per molecule, being more hydrophilic, interacts favorably with cotton than polyester in the hydrophobic environment. The adsorption of the pure surfactants on model gold surface was also established using Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) system. Effect of ethylene oxide chain length and surfactant concentration on the extent of adsorption was precisely quantified and adsorption isotherms were constructed. Adsorption was studied from both aqueous solutions and solutions in hydrophobic solvent of the surfactants. Information about thickness and structure of adsorbed layer was calculated from the QCM-D frequency-dissipation data.